109 people were detained on Friday.
130 people have been detained for urinating in the open in and around Agra and fined Rs 100 to Rs 500.
The Government Railway Police (GRP) carried out the crackdown over the past three days in railway station areas in Agra, Agra Fort, Agra Cantonment, Mathura and Tundla as part of the Swachch Bharat Mission.
109 people were rounded up on Friday and spent a day in detention.
On Saturday, another 21 were detained. They spent 8-10 hours in a lock-up and tendered written apologies.
GRP's Superintendent of Police Gopesh Nath Khanna said the campaign would continue.
"People have this bad habit of relieving themselves just about anywhere, without bothering about public hygiene," a railway official said.
Agra has half a dozen railway stations and important trains pass through the city. But travellers and residents complain that the civic bodies pay no attention to cleaning up areas outside the stations.