The instruction came after television channels aired images of the youth carrying the dead body.
A man was forced to tie the body of his 18-year-old younger brother to his bicycle and take it home on Tuesday from a hospital in Majuli, one of the most underdeveloped parts of Assam.
In one of the many tragic replays of the story last year of Dana Majhi - who walked to his village carrying his dead wife on his shoulder - the man was seen in visuals on TV channels walking with his brother's body on a bamboo bridge that is the only link between his village and the town.
Jolted by the visuals from the constituency that he represents in the state assembly, Chief Minister Sarbananda Sonowal has ordered an investigation.
Majuli, the largest river island in Assam, is also part of the Lok Sabha constituency that Mr Sonowal represented before becoming Chief Minister last year.
Ambulances do not run in this region because of the absence of a proper bridge or motorable road.
The man told reporters that he took his brother to the hospital for urgent treatment but he died.
The hospital claims the man refused to wait even when he was told that an ambulance was being arranged.
"The patient was brought to the hospital on Tuesday morning by his relative. However, he died while we were preparing to put him on oxygen and start the treatment. We told the relative to wait so that we can organise an ambulance but the relative did not listen and tied the body to his cycle and took it back," said a hospital official.
In August, the country was shocked to see images of Dana Majhi carrying his wife's body slung over his shoulder to reach his village in Odisha's Kalahandi district after being denied help from the hospital authorities.
In Bihar, two policemen were suspended for tying a rope around a dead man's neck and dragging the body from the Ganga river bank in the absence of an ambulance.
(With inputs from IANS)