The pop diva and her dancers performed other chartbusters, including "Single Ladies," "Naughty Girl," "Halo," "Survivor" and "Baby Boy," as well as "Run The World (Girls)."
The singer also performed Etta James' "At Last," with giant images of US President Barack Obama flashing on screens behind her. Beyonce had performed to this song at President Obama's Inaugural Ball in 2009.
Bono, lead singer of U2, one of the most popular and influential rock bands of recent times, performs on the Pyramid stage during the Glastonbury festival.